IMPROVE YOUR CRISPR-Cas9 GENOME EDITING EXPERIMENTS

During this presentation you will learn about:

  • • Current strategies for CRISPR-Cas9 mediated genome editing with emphasis on commonly used methods for delivery of RNA triggers and Cas9 protein.
  • • Considerations for the design of guide RNAs and donor templates intended for homology-directed repair.
  • • Common errors that can occur during the CRISPR workflow and how to avoid them.
Date: Thursday, March 31, 2016
Time: 12:00–1:00 pm
Location: University of California, Berkeley
Address: Energy Bioscience Building Room 115, Energy Biosciences Institute

Lunch will be provided

About the presenter

photo of Justin Barr

Justin Barr

Scientific Applications Specialist

Justin Barr is a Senior Scientific Applications Specialist at IDT, where he assists researchers with experimental design and support. Justin has a BS in Biology from the University of Iowa, with an emphasis in genetics and biotechnology. He is also currently pursuing an MBA at the Tippie School of Management at the University of Iowa. Prior to joining IDT in 2013, Justin worked for six years in the laboratory of Dr. David Motto at the University of Iowa, studying the effects of VWF and ADAMTS13 on endothelial dysfunction.

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Alt-R™ CRISPR-Cas9 System

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“After trying a variety of solutions, the IDT Alt-R CRISPR-Cas9 System was the most user friendly, and it generated superior results in our assays.”

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“The IDT modified crRNAs (Alt-R CRISPR-Cas9 System) work well in our assay and can generate fully penetrant phenotypes in transfected mammalian cells. They have allowed us to rapidly validate hits from pooled sgRNA screens without the need to construct expression plasmids or to generate lentivirus.”

—Large pharmaceutical customer