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Have 2 standard desalted DNA oligonucleotides premixed in a single tube according to your specifications. This can be useful when performing PCR, fluorescent dye RT-PCR, or when generating sets of insertions or deletions through site-directed mutagenesis.

Do my oligos need to be purified?

Do your oligos need purification for your intended application? Use these recommendations based on oligo length, application, yield required, and presence of modifications to determine which oligonucleotide purification method to select.

Calculations: Converting from nanograms to copy number

Here is a calculation often used when creating a qPCR standard curve. Link to this free, online tool that will do it for you.

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custom oligos • qPCR • next generation sequencing • RNAi • genes & gene fragments • CRISPR genome editing

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