Breaking down qPCR curves

Access expertise that will improve your data

This special 3-part edition of Real-time PCR guide: Design, validation, analysis, and troubleshooting will familiarize you with critical parameters in assay design. Attention to these factors comes first when designing experiments that yield quantitative, accurate, and interpretable data. Understand these factors and you will soon be setting up experiments that generate great results, reliably. In addition, a series of stylized amplification curves representing commonly experienced qPCR issues is provided, along with causes and solutions. Matching up your data with one of these images will set you on the path to correcting the problems, and avoiding them in the future.