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Webinar: An improved solution for generating large CRISPR HDR knock-ins

Available on-demand

CRISPR-based homology-directed repair (HDR) is an invaluable tool to facilitate specific mutations in a genomic region of interest in research studies. While many methods have been reported for improving HDR efficiency, achieving precise changes via HDR remains a challenge, particularly for large knock-ins. These insertions can be generated via HDR using enzymatically-generated donor templates. In this webinar you will learn:

  • How dsDNA donor templates with novel end-modifications improve the frequency of HDR and reduce homology-independent (blunt) insertion events.
  • How the addition of Alt-R HDR Enhancer V2, a small molecule compound, can further improve HDR rates.
  • Design considerations when using large dsDNA templates for successful HDR experiments.


Fill out the form below to view the webinar on-demand.

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Presenters

Jessica Woodley, MS
Jessica Woodley, MS

Research Scientist
Molecular Genetics Research Group
IDT

Jessica Woodley is a research scientist in the molecular genetics research group at IDT. Jessica earned her Master's degree in Microbiology from the University of Iowa, where her research focused on Staphylococcus aureus biofilm formation. Since joining IDT in 2016, her work has focused on the development of rhPCR technologies and improved methods for efficient homology-directed repair in CRISPR applications.

Mollie Schubert, MS
Mollie Schubert, MS (Moderator)

Research Scientist
Molecular Genetics Research Group
IDT

Mollie Schubert is a research scientist in the Molecular Genetics Research Group at Integrated DNA Technologies. Mollie received her Master's degree in Biochemistry from Iowa State University, and has been with IDT since 2013. For the past five years, Mollie's studies have focused on CRISPR gene editing. Her research has included high-throughput screening of CRISPR-Cas9 guides for the development of a site selection tool, optimization of composition and delivery for synthetic RNA reagents complexed to recombinant CRISPR nucleases, and the development of methods for efficient gene editing, with emphasis on homology-directed repair.