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Browse our library of PhD-written protocols for CRISPR applications. If you still need assistance with your experiments, an IDT CRISPR expert would be happy to help.

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Vertical spacers are responsive and 50% smaller on mobile than on tablet+.
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Vertical spacers are responsive and 50% smaller on mobile than on tablet+.

Homology-directed repair using the Alt-R CRISPR-Cas9 System and HDR Donor Oligos

Cationic lipid delivery of CRISPR RNP complexes into mammalian cells

In vitro cleavage of target DNA with RNP complexes

RNP delivery into mouse zygotes via electroporation

RNP microinjection of mouse zygotes

RNP preparation for fruit fly embryo microinjection

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Genome Editing Detection Kit Protocol

HDR via simultaneous delivery of RNP complexes and dsDNA repair templates using the Lonza Shuttle Nucleofector System

HDR using CRISPR-Cas9 and HDR Donor Oligos in primary T cells

Microinjection of zebrafish embryos with RNPs

Electroporation of primary human CD34+ hematopoietic stem and progenitor cells

HDR via CRISPR-Cas9 nickase

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Delivery of RNP complexes into HEK-293 cells using the Lonza Nucleofector System

HDR using CRISPR-Cas9 and HDR Donor Oligos in iPSCs

RNP delivery into Jurkat T cells using the Bio-Rad Gene Pulser Xcell Electroporation System

FACS analysis of RNP transfected cells via fluorescently labeled tracrRNA

Gene knockin via HDR reference guide

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Delivery of RNP complexes into Jurkat T cells using the Neon Transfection System

CRISPR gene knockout from design to analysis

RNP electroporation of human pluripotent or embryonic stem cells

RNP electroporation of human B cell lines

C. elegans RNP injection

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