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Webinar: How to optimize CRISPR genome editing in primary T cells

Available on-demand

Genome editing in primary T lymphocytes has unique challenges that make generating correct clones a significant bottleneck in the progression of research. This webinar will present optimized methods for CRISPR editing in primary T cells including optimized cell culture and delivery parameters for high-efficiency editing. Additionally, we will discuss methods for improving knock-in rates with both short and long inserts through optimized design of HDR donors and the use of HDR enhancing reagents.


In this webinar, you will learn how to:

  • Define different double-strand break repair pathways and utilize them for CRISPR editing
  • Carry out your own CRISPR editing experiments in primary T cells
  • Achieve high-efficiency knock-in rates in primary T cells with optimized methods and reagents


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Fill out the form below to register.

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Presenters

Bernice Thommandru, MS
Bernice Thommandru, MS

Research Scientist, Molecular Genetics Research Group, IDT

Bernice Thommandru is a research scientist in the Molecular Genetics research group at IDT. She received her MS in Molecular Physiology and Biophysics from the University of Iowa, where she studied transcriptional regulation of multi-drug resistance in pathogenic fungi of the lung. At IDT, Bernice has focused on optimizing delivery strategies for CRISPR reagents as well as methods for increasing the rate of homology-directed repair.

Mollie Schubert, MS
Mollie Schubert, MS

Research Scientist, Molecular Genetics Research Group, IDT

Mollie Schubert is a research scientist in the Molecular Genetics Research Group at IDT. Mollie received her Master's degree in Biochemistry from Iowa State University, and has been with IDT since 2013. For the past five years, Mollie's studies have focused on CRISPR gene editing. Her research has included high-throughput screening of CRISPR-Cas9 guides for the development of a site selection tool, optimization of composition and delivery for synthetic RNA reagents complexed to recombinant CRISPR nucleases, and the development of methods for efficient gene editing, with an emphasis on homology-directed repair.