SIMPLIFIED GENOME EDITING OF PRIMARY CELLS WITH OPTIMIZED CRISPR RIBONUCLEOPROTEIN DELIVERY

Increasing evidence shows that the most efficient CRISPR-based genome editing occurs after delivery of CRISPR reagents as ribonucleoprotein (RNP) complexes. Whether you are working with electroporation or lipid-based transfection methods, we can help you plan and execute your experiments. In particular, we will cover tips for:

  • Handling of clinically relevant primary cell cultures
  • Performing a plasmid-free workflow using the Alt-R™ CRISPR-Cas9 System, including how to design guide RNAs and analyze CRISPR mutants
  • Using fluorescently labeled tracrRNA during experimental planning or CRISPR mutant analyses
Date:
Friday, May 12, 2017
Time:
10:30 am–noon
Location:
University of Cologne, CMMC Seminar Room

Presenters

Christiane Thanish

Christiane Thanisch, PhD

Scientific Application Manager

Brian Caudill

Brian Caudill

Territory Manager

What our customers say

“After trying a variety of solutions, the IDT Alt-R CRISPR-Cas9 System was the most user friendly, and it generated superior results in our assays.”

—Large pharmaceutical customer

“The IDT modified crRNAs [Alt-R CRISPR-Cas9 System] work well in our assay and can generate fully penetrant phenotypes in transfected mammalian cells. They have allowed us to rapidly validate hits from pooled sgRNA screens without the need to construct expression plasmids or to generate lentivirus.”

—Large pharmaceutical customer