Tips for designing HDR donor templates

CRISPR-mediated HDR offers world-changing potential, but also comes with complex practical obstacles. When we hear from researchers whose experiments suffer from low and inconsistent HDR efficiencies, we always point to our Megamer ssDNA Fragments.

These high-fidelity, sequence-verified, single-stranded DNA strands come in lengths of 201–2000 bases, and are ideal ssODNs for HDR. They have helped steer the ship for countless high-impact projects, and we've compiled our "Top 5” tips for their use.

Download your PDF and start doing better CRISPR today!