Optimizing for CRISPR-Cas9 homology-directed repair

A new application note

Starting with a summary of the key concepts involved in CRISPR-Cas9–mediated HDR, this application note explores the optimization of HDR in mammalian cell lines. We discuss criteria for selecting guide RNA sequences, highlight considerations for rational design of synthetic DNA donor templates, and detail how efficient HDR can be achieved by optimizing each step of the experimental procedure.

What our customers say

“After trying a variety of solutions, the IDT Alt-R CRISPR-Cas9 System was the most user friendly, and it generated superior results in our assays.”

—Large pharmaceutical customer

“The IDT modified crRNAs [Alt-R CRISPR-Cas9 System] work well in our assay and can generate fully penetrant phenotypes in transfected mammalian cells. They have allowed us to rapidly validate hits from pooled sgRNA screens without the need to construct expression plasmids or to generate lentivirus.”

—Large pharmaceutical customer