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Webinar: How to optimize non-viral CRISPR HDR for high-efficiency large knock-in in primary T cells and iPSCs

Available on-demand

Achieving large knock-ins, such as chimeric antigen receptor (CAR) insertions in primary T lymphocytes, remains a key challenge in genome editing. In this webinar, we will present our findings from extensive bench research focused on optimizing CRISPR homology-directed repair (HDR) techniques in CAR knock-ins in primary T cells. We will cover crucial factors including double strand break repair mechanisms, CRISPR reagent selection, and optimized cell handling workflows. Additionally, we will highlight the use of Aldevron Nanoplasmids™ as highly efficient HDR donor templates, successfully demonstrated in both primary T cells and induced pluripotent stem cells (iPSCs). The session will conclude with a workflow summary and a Q&A session.


After this webinar, participants will be able to:

  1. Understand principles of CRISPR: Gain a foundational understanding of CRISPR by comparing non-homologous end joining (NHEJ) and homology-directed repair (HDR).
  2. Optimize primary T cell workflows: Learn best practices for activating and culturing primary T cells to enhance CRISPR editing efficiency.
  3. Apply Nanoplasmids in HDR: Explore the benefits and optimization strategies for using Aldevron Nanoplasmids™ as donor templates for large insertions in CRISPR-mediated HDR.
  4. Leverage HDR Enhancers: Learn how HDR enhancing reagents can boost editing success rates in challenging applications.

Fill out the form below to register.

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Presenters

Bernice Thommandru, MS
Bernice Thommandru, MS

Research Scientist, Molecular Genetics Research Group, IDT

Bernice Thommandru is a research scientist in the Molecular Genetics research group at IDT. She received her MS in Molecular Physiology and Biophysics from the University of Iowa, where she studied transcriptional regulation of multi-drug resistance in pathogenic fungi of the lung. At IDT, Bernice has focused on optimizing delivery strategies for CRISPR reagents as well as methods for increasing the rate of homology-directed repair.

Tyler Kozisek, PhD
Tyler Kozisek, PhD

Senior Scientist, Aldevron

Tyler Kozisek is a Senior Scientist at Aldevron in the research and development department. He received his bachelor’s degree in Mechanical Engineering and PhD in Biomedical Engineering from the University of Nebraska – Lincoln. Tyler's PhD work focused on enhancing nonviral gene delivery platforms for transfection of primary stem cells. Tyler joined Aldevron in March of 2022 as an R&D Scientist and focuses on designing Nanoplasmids™ vectors for clients, as well as advancing the Nanoplasmid platform.