It’s not you, it’s your qPCR assay

Troubleshooting expertise that will improve your qPCR data

Despite your careful attention to assay design and experimental parameters, analysis of your results might reveal suboptimal data. In this special application guide, Real-time qPCR guide: Part 3—Troubleshooting, we provide a series of stylized amplification curves representing commonly experienced qPCR issues. Each comes with a list of causes and reference to a section in the guide discussing solutions. Match up your data with one of these images and you will find a list of causes and discussion of how to avoid them.

If you missed receiving our application guides for assay design (Part 1) and assay validation and data analysis (Part 2), access them from the DECODED article, Ensure reliable qPCR results—advice on assay validation and data analysis.)