It’s not you, it’s your qPCR assay design

Expertise that will improve your qPCR data

Poor qPCR assay design—imperfect hybridization conditions, presence of underlying SNPs, or challenging target sequence region—can lead to suboptimal amplification and extensive troubleshooting.

Even use of previously published assays that weren’t validated under your specific reaction conditions can require repeated runs, wasting valuable time and resources, including precious samples.

This special edition Real-time qPCR guide: Part 1—Assay design will familiarize you with critical variables in assay design. Attention to these factors is the first step to ensure your qPCR experiments provide quantitative, accurate, and interpretable data.